caspase dependent apoptosis induced by cladribine in the estrogen receptor negative breast cancer cell line, mda-mb468

نویسندگان

f. karami-tehrani

cancer research laboratory, department of clinical biochemistry, school of medical sciences, university of tarbiat modarres, tehran, islamic republic of iran

چکیده

the purpose of the present study is to investigate the cytotoxicity/apoptotic effect of 2-chloro-2′-deoxyadenosine, cladribine, (2-cda) in the human breast cancer cell line, mda-mb468 (estrogen receptor negative, er−). mtt [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h tetrazolium bromide] assay, annexin v-fluorescein/pi and hoechst 33258 staining were used to detect cytotoxicity and cell apoptosis. the activation of caspase-3 and -9 was assayed using caspase activation assay kits. gel electrophoresis was performed to detect dna fragmentation. treatment of mda-mb468 cells with different concentrations of 2-cda (50, 100 and 500 µm) resulted in a significant increase in the cell death. annexin v-fluorescein/pi and hoechst 33258 staining revealed that the cell death was mainly of apoptotic type. dna laddering profile was also obtained in the treated mda-mb468 cells using dna fragmentation analysis. a significant (p

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منابع مشابه

CASPASE DEPENDENT APOPTOSIS INDUCED BY CLADRIBINE IN THE ESTROGEN RECEPTOR NEGATIVE BREAST CANCER CELL LINE, MDA-MB468

The purpose of the present study is to investigate the cytotoxicity/apoptotic effect of 2-chloro-2′-deoxyadenosine, cladribine, (2-CdA) in the human breast cancer cell line, MDA-MB468 (estrogen receptor negative, ER−). MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide] assay, annexin V-Fluorescein/PI and Hoechst 33258 staining were used to detect cytotoxicity and cell apopto...

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caspase dependent apoptosis induced by cladribine in the estrogen receptor negative breast cancer cell line, mda-mb468

the purpose of the present study is to investigate the cytotoxicity/apoptotic effect of 2-chloro-2′-deoxyadenosine, cladribine, (2-cda) in the human breast cancer cell line, mda-mb468 (estrogen receptor negative, er−). mtt [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h tetrazolium bromide] assay, annexin v-fluorescein/pi and hoechst 33258 staining were used to detect cytotoxicity and cell apopto...

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ADENOSINE DEAMINASE ACTIVITY IN ESTROGEN RECEPTOR POSITIVE AND NEGATIVE HUMAN BREAST CANCER CELL LINES

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عنوان ژورنال:
journal of sciences islamic republic of iran

جلد ۱۴، شماره ۴، صفحات ۳۰۳-۳۱۰

کلمات کلیدی
[ ' c l a d r i b i n e ' , ' a p o p t o s i s ' , ' m d a ' , ' m b 4 6 8 ' , ' c a s p a s e ' , 3 , ' c a s p a s e ' , 9 ]

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